OPTIMIZATION OF CULTURE MEDIA CONTENT FOR ACCELERATION OF GROWTH AND CULTIVATION OF ROSA DAMASCENA MILL. IN IN VITRO CULTURE

O.О. Oliynyk, A.А. Kluvadenko, M.D. Melnychuk

Abstract


The analysis of direct morphogenesis of Rosa damascena Mill. was performed on varieties of Ukrainian selection of Lan', Lada and Raduga. It was established that roses microclonally propagate more effectively by using apical meristems of vegetative buds (Lada, Lan'), or by stem explants (Raduga, Lan'). For the chosen samples we optimized the contents of the nutrient media that provide growth acceleration. For Lan' variety, it is recommended to cultivate regenerated plants on MS or QL culture media, without adding growth hormones, for Raduga variety – Anderson medium with doubled quantity of Fe-chelate and 2,0 mg·l-1 BAP, for lada variety – Anderson medium with addition of 0.5 mg·l-1 BAP or WPM 1.5 mg·l-1BAP, 0.1 mg·l-1 NAA.

Keywords


Rosa damascena Mill.; culture medium; in vitro culture; direct morphogenesis; explant

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DOI: https://doi.org/10.15421/40260721

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