FEATURES OF STERILIZATION AND INTRODUCTION TO THE CULTURE IN VITRO OF EXPLANTS OF TAXUS BACCATA L.

M.М. Lisoviy

Abstract


The critical analysis and a brief review of the literature concerning the studied subjects. Described in detail the methodology of the research applied: the initial plant material used as explants in micropropagation of Taxus baccata L.; sequence use of chemical reagents, their concentration and duration of soaking in sterilization; types and modifying nutrient media. An optimal scheme of decontamination initial plant material studied species and found optimal composition environment and phytohormones combination for a successful initiation explants. Summarized and analyzed the results.

Keywords


Taxus baccata L.; in vitro; explants; sterilization; initiation; nutrient medium decontamination

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