THE DIRECT ORGANOGENESIS OF POPULUS TREMULA L. FROM DIFFERENT TYPES OF EXPLANTS IN VITRO CULTURE

S.Yu. Bilous

Abstract


The features of direct induction of tissue regeneration of P. tremula depending on genotype, type of explants and culture conditions in vitro were researched. The ways of implementation of the morphogenetic potential of microshoots P. tremula to form rooted plants were established. Some approaches to microclonal reproduction of aspen were elaborate. The optimal components of the culture medium with the addition of 0.25-0.5 mg∙l-1 kinetyn, tidiazuron and activated carbon, which provided the full realization of morphogenetic potential to form explants rooted plants and the possibility of long-term cultivation P. tremula in conditions in vitro are sampled.

Keywords


Populus tremula L.; in vitro; the culture medium; explants; morphogenesis; growth regulators

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