HETEROBASIDION ANNOSUM ROOT ROT INFECTION DEVELOPMENT IN SCOTS PINE AND EVALUATION OF THE EXPRESSION LEVELS OF LIPID TRANSFER PROTEIN AND DEFENSINS IN INFECTED TISSUES

N.I. Hrunyk, Yu.M. Yusypovych, V.A. Kovaleva, R.T. Gout

Abstract


Heterobasidion annosum is a causative agent of the root and butt rot and appears to be one of the most economically important conifer pathogens, which causes havoc in boreal forests at northern hemisphere. Despite biology and genetics of this fungus is well studied, the mechanisms of defense responses and resistance of pine trees are still unclear. Recent advances in transcript profiling and molecular characterization of pathogenicity factors approached us in our understanding of this system. In this study we showed features of the development of pathological process in Scots pine saplings caused with annosum root rot at different periods of their growth and changes in expression levels of a lipid transfer protein and defensins by semi QRT-PCR.

Keywords


Scots pine; defensin; lipid transfer protein; expression; annosum root rot

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