SOME FEATURES OF GETTING ASEPTIC CULTURE OF ACER SACCHARINUM L. IN VITRO

S.Yu. Bilous, O.M. Kurdyuk, O.V. Medvedchuk

Abstract


The actuality of microclonal propagation of Acer Saccharinum L. is motivated. The features of morphogenesis of Acer Saccharinum L. on the stage of getting sterile culture in vitro are presented. The optimal period, type of explants, concentration of liquid and time for effective sterilisation (95 %) and getting aseptic culture of Acer saccharinum L. ae obtained. Main components of nutrition medium with adding cytokinin activate plant grow regulators are proposed. The most effective nutrition medium using 0.2 mg∙l-1 TDZ for successfully obtaining of active morphogenic micro shoots is selected.

Keywords


Acer Saccharinum L.; microclonal propagation; explants; culture medium; plant regenerants; morphogenesis; in vitro

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