SELECTION OF STERILIZER, INTRODUCTION TO THE CULTURE AND PROPAGATION OF PLANT MATERIAL OF AILANTUS ALTISSIMA (MILL.) SWINGLE SPECIES

V.V. Mamchur

Abstract


The purpose of the research was to study features of microclonal reproduction of Ailanthus altissima (Mill.) Swingle in conditions in vitro, sterilization of plant material, selection and modification of nutritious environment. Studies are conducted in the laboratory of microclonal reproduction of Uman National University of Horticulture. As primary explants the authors used A. altissima twigs 1.0-1.5 cm long. Material was taken from the National Arboretum "Sofiyivka" NAS of Ukraine from plants that have not reached the generative stage during active growth of the shoots. The process of microclonal A. altissima plant propagation in vitro in culture includes several successive stages, namely: sterilization of plant material; introduction to culture in vitro; selection and optimization of the nutritious environment; receiving plants regenerants. Before sterilization shoots with leaves were removed, after that the plant material was washed in running tap and distilled water, wiped gauze cloth soaked in 70 % ethanol th. Prepared explants were placed in sterile dishes and treated sequentially with solutions of sterilizing agents. Sterilization was carried out in several stages (preliminary and main). After sterilization explants were washed three times with sterile distilled water (15 min.) And planted them in our modified nutritious environment. Within 7 days each option was determined by the effectiveness of sterilization, sterile and calculating the percentage of infected explants. Viability entered explants were evaluated after 25 day period. This defeat of fungal and bacterial infections was small (10 %) and necrosis of plant tissues were observed. Evaluating the effectiveness of environment and record multiplication factor was carried out after the second passage. To concluse, the dependence of explants morphogenesis on the hormonal composition of the nutritious environment is defined. Numerous studies have enabled selecting the optimum environment that provided the multiplication factor of more than two. As a result of studies we have found that in the environment MS3 (BAP – 0.5 mg/l IAA – 0.1 mg/l) and MS5 (BAP – 0.5 mg/l NOC – 0.1 mg/l) multiplication factor amounted to 7.35 % and 5.05 % respectively. In the environment MS7 with the addition of 0.8 mg/l BAP and 0.3 mg/l IMC we observed maximum growth of micro shoots, high morphogenic capacity, increasing the number of internodes and multiplication factor amounted to 8.5 %.

Keywords


explants; sterilizers; culture medium; morphogenesis; growth regulators

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References


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DOI: https://doi.org/10.15421/40270412

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