BIOTECHNOLOGICAL ASPECTS OF MICROPROPAGATION OF PLANTS OF THE GENUS SALIX L.

О.Yu. Сhornobrov

Abstract


The aseptic conditions for obtaining viable explants of three species and one cultivar of the genus Salix L. isolated from donor plants in different phenophase were established. The optimum composition of the culture media for microclonal propagation, rooting and receiving regenerant plants was selected. The biotechnology of microclonal propagation of abovementioned plants, including the selection of the culture media components for various genotypes, stages and explants types, was developed. A significant number of in vitro regenerants for a different purposeful use were obtained by using activation of growth of existing explants meristem and by means of direct and indirect morphogenesis.

Keywords


Salix L.; culture in vitro; explants; micropropagation; callus; basal medium; regenerant plants

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DOI: https://doi.org/10.15421/40260727

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